Amounts and available modifications

A set of hybridization probes consists of a pair of oligonucleotide probes which can hybridize neighbored on a DNA template, one labeled with fluorescein at its 3'-terminus [3FL] and the other labeled at its 5'-end with LightCycler® Red dyes [5LC]. The free 3'-hydroxy group will usually be blocked with a phosphate to prevent extension by the polymerase. A gap of one to four bases (4 to 25 Å distance) between the probes is recommended.

Code

Product

Amount 

Tests 

Label

17-0640-01

Custom Synthesis 5'-LC640,
3'-Phosphate

1 nmol

300

5LC 1.0

17-0640-03

Custom Synthesis 5'-LC640,
3'-Phosphate

3 nmol

1.000

5LC 3.0

17-0640-06

Custom Synthesis 5'-LC640,
3'-Phosphate

2 x 3 nmol

2.000

5LC 2x3

17-0640-30

Custom Synthesis 5'-LC640,
3'-Phosphate

30 nmol

10.000

5LC 30

17-0640-33

Custom Synthesis 5'-LC640,
3'-Phosphate

10 x 3 nmol

10.000

5LC 30

17-0644-01

Custom Synthesis int. dT-C2-LC640, 3'-OH

1 nmol

300

iLC 1.0

17-0644-03

Custom Synthesis int. dT-C2-LC640, 3'-OH

3 nmol

1.000

iLC 3.0

17-0644-30

Custom Synthesis int. dT-C2-LC640, 3'-OH

30 nmol

10.000

iLC 30

17-0705-01

Custom Synthesis 5'-LC705,
3'-Phosphate

1 nmol

300

5LC 1.0

17-0705-03

Custom Synthesis 5'-LC705,
3'-Phosphate

3 nmol

31.000

5LC 3.0

17-0705-06

Custom Synthesis 5'-LC705,
3'-Phosphate

2 x 3 nmol

2.000

5LC 2x3

17-0705-30

Custom Synthesis 5'-LC705,
3'-Phosphate

30 nmol

10.000

5LC 30

17-0705-33

Custom Synthesis 5'-LC705,
3'-Phosphate

10 x 3 nmol

10.000

5LC 30

17-2121-01

Custom Synthesis 3'-Fluorescein

1 nmol

300

3FL 1.0

17-2121-03

Custom Synthesis 3'-Fluorescein

3 nmol

1.000

3FL 3.0

17-2121-06

Custom Synthesis 3'-Fluorescein

2 x 3 nmol

2.000

3FL 2x3

17-2121-30

Custom Synthesis 3'-Fluorescein

30 nmol

10.000

3FL 30

17-2121-33

Custom Synthesis 3'-Fluorescein

10 x 3 nmol

10.000

3FL 30

17-2640-00

HybProbe 640 Set LC640/3FL

per 300 pmol

100

0.3

17-2705-00

HybProbe 705 Set LC705/3FL

per 300 pmol

100

0.3

Delivered amounts and assays

HybProbes are available in the following product amounts: 1 nmol, 3 nmol and 30 nmol. The 300 pmol scale is for evaluation of the system and is only available as a set. Other scales are available upon request. The prices include the synthesis of the oligonucleotides (30 bases). All products are purified by a multistep HPLC procedure and desalting. Delivery condition is lyophilized. Numbers of tests were calculated assuming the use of 3 pmol per assay. In routine, this amount may vary between 2 pmol (0.1 µM) and 10 pmol (0.5 µM) probe per assay.

300 pmol

1 nmol

3 nmol

2 x 3 nmol

30 nmol

100 Tests

300 Tests

1.000 Tests

2.000 Tests

10.000 Tests